Method for assay of Terbinafine HCl Present in topical Cream Formulation was developed and validated by RP-HPLC method. Testing was done by different Analytical columns such as C8, CN and C18 column. Finally good separation was achieved using Phenomenex C18 (250mm x 4.6mm, 5µ) used for analysis. The analysis time was 30 min and flow rate was 1 ml/min. Mobile phase used for chromatography was filtered and degassed mixture of Acetonitrile: Methanol: Water (40:10:50), 0.1 ml O-Phosphoric Acid and 0.1 ml of Triethalamine. UV detection was accomplished at 282 nm. The method was validated according to ICH guideline recommendation. The report demonstrates that this method is effective, simple, sensitive and Accurate for Assay of Terbinafine HCl in cream formulation.
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